An essay about the Nipah virus.

Infectious Disease

A sample has been sequenced from an epidemic outbreak from an unknown illness that is going around.

The sequence can be found at the end of this paper.

Part A.

Generate an alignment of related sequences, you must have at least 20 sequences in your alignment.

Generate a phylogenetic tree of the alignment that will enable you to identify the nearest neighbour of the sequence and any related species.

Part B.

Each question should be no more than 1000 words.

1. Write a section describing the viral family described, include replication of the virus and transmission.
2. What is the history of the virus that you have identified? Include a description of the previous outbreaks.
3. Given that you have a patient sick in the hospital, what measures would you take to try and control this new outbreak?

Sequence of unknown etiology:

ATGAGTGATATCTTTGAAGAGGCGGCTAGTTTTAGGAGTTATCAATCTAAGTTAGGGAGAGATGGGAGAG

CTAGTGCAGCAACTGCTACTTTGACAACCAAGATAAGGATATTTGTACCAGCTACTAATAGTCCAGAGCT

CAGATGGGAACTAACATTGTTTGCACTTGATGTGATTAGATCTCCGAGTGCTGCCGAGTCAATGAAAGTT

GGAGCTGCTTTCACACTCATCTCTATGTATTCAGAGAGACCCGGGGCTCTCATTAGAAGTCTCCTCAATG

ACCCAGACATTGAAGCTGTAATAATAGATGTTGGATCAATGGTCAATGGAATACCAGTAATGGAGAGGAG

AGGAGACAAGGCTCAGGAGGAGATGGAAGGCTTGATGAGAATCCTCAAAACTGCTCGGGACAGCAGCAAG

GGAAAGACACCTTTTGTTGACAGCCGAGCTTACGGCCTACGGATAACAGACATGAGCACCCTGGTCTCTG

CAGTTATCACCATCGAGGCCCAGATCTGGATACTGATCGCTAAAGCAGTTACAGCTCCCGACACTGCCGA

GGAAAGTGAAACTAGAAGATGGGCTAAATACGTCCAACAAAAGAGAGTCAATCCGTTCTTTGCTCTAACT

CAGCAATGGCTAACAGAAATGAGGAATCTGCTCTCCCAGAGTCTATCAGTAAGGAAGTTCATGGTTGAGA

TCCTCATAGAAGTCAAGAAAGGAGGATCTGCTAAAGGCAGAGCAGTAGAAATAATCTCAGACATCGGAAA

CTATGTCGAGGAAACTGGTATGGCAGGATTCTTCGCAACCATCAGATTCGGGTTGGAGACAAGGTATCCA

GCACTTGCACTCAACGAATTCCAGAGTGACCTCAACACCATCAAAAGCTTGATGCTACTCTACAGAGAAA

TTGGCCCAAGAGCCCCTTATATGGTGCTTCTTGAAGAATCAATTCAGACTAAATTTGCCCCTGGAGGTTA

CCCATTATTGTGGAGCTTTGCCATGGGTGTGGCTACTACTATTGACAGGTCTATGGGGGCATTGAATATC

AATCGTGGTTATCTTGAGCCTATGTATTTCAGACTAGGCCAAAAATCAGCACGTCACCATGCTGGAGGAA

TTGATCAGAACATGGCAAATAGACTGGGACTAAGTTCAGATCAAGTTGCAGAACTCGCTGCTGCAGTTCA

GGAAACATCAGCAGGAAGACAAGAGAGTAACGTTCAGGCTAGAGAGGCAAAATTTGCTGCAGGAGGTGTG

CTCATTGGAGGCAGTGATCAAGATGTCGATGAAGAGGAAGAACCTATAGAACAGAGTGGCAGACAGTCAG

TTACCTTCAAAAGGGAGATGAGTATTTCATCCCTCGCTGACAGTGTGCCGAGCAGTTCTGTGAGCACATC

CGGTGGGACCAGATTGACCAATTCATTACTAAACCTCAGATCAAGACTGGCTGCAAAAGCAGCAAAAGAA

GCCGCCTCATCCAATGCAACAGATGATCCAGCAATCAGCAACAGAACTCAAGGGGAATCAGAGAAGAAGA

ATAATCAAGACCTCAAACCTGCTCAAAATGACCTTGATTTCGTCAGAGCTGATGTGTGAATGGATAAATT

GGAACTAGTCAATGATGGCCTCAATATTATTGACTTTATTCAGAAGAACCAAAAAGAAATACAGAAGACA

TACGGACGATCAAGCATTCAACAACCCAGCATCAAAGACCGAACAAAAGCCTGGGAAGATTTTCTGCAGT

GCACCAGTGGAGAATCTGAACAAGTTGAGGGGGGAATGTCTAAGGATGATGGAGATGTTGAAAGAAGAAA

CTTGGAGGATCTATCCAGTACTTCTCCCACAGATGGAACTATTGGAAAGAGAGTGTCGAACACCCGTGAC

TGGGCAGAAGGTTCAGATGACATACAACTGGACCCAGTGGTTACAGACGTTGTATACCATGATCATGGAG

GAGAATGTACCGGATATGGATTTACTTCAAGCCCTGAGAGAGGGTGGAGTGATTACGCATCAGGAGCAAA

CAATGGGAATGTATGTCTTGTATCTGATGCAAAGATGCTGTCCTATGCTCCCGAAATTGCAGTTTCTAAA

GAAGATCGGGAAACTGATCTAGTTCATCTTGAGAATAAACTATCTACTACAGGACTGAATCCCACAGCAG

TACCATTCACTCCGAGAAACCTGTCTGATCCTGCAAAAGACTCTCCTGTGATTGCTGAACACTACTACGG

ACTAGGAGTTAAAGAGCAAAACGTTGGCCCTCAGACTAGCAGAAATGTCAATTTGGACAGCATCAAATTG

TACACATCAGATGACGAAGAGGCAGATCAGCTTGAATTCGAAGATGAGTTTGCAGGAAGCTCAAGTGAAG

TAATAGTCGGCATTTCTCCTGAAGATGAAGAGCCTTCAAGTGTCGGCGGAAAACCCAATGAATCCATTGG

ACGTACAATCGAAGGCCAATCAATCCGAGACAGCCTTCAAGCCAAGGACAACAAATCAGCAGATGTACCA

GGAACAGGACCGAAAGATTCAGCAGTGAAGGAAGAACCACCCCAGAAGAGGCTACCTATGTTAGCTGAAG

AATTTGAGTGCTCTGGATCGGAAGACCCAATCATTCGAGAGCTGCTGAAGGAGAACTCACTCATAAATTG

TCAGCAAGGGAAAGATGCTCAGCCTCCATATCATTGGAGCATCGAGAGGTCAATAAGCCCGGATAAGACT

GAGATCGTCAACGGTGCTGTGCAAACTGCTGACAGGCAAAGACCAGGAACTCCGATGCCAAAGTCCCGAG

GTATTCCCATTAAAAAGGGGGGGGACACGAAAAATCCATCTGCTGGGACGGAAAAAATGCCTGGGTCGAA

GAGAGGTGGAACCCGGCCTGTTCGAGGATCACCCCCCCCCCCCCAAAGCAAGAGTGTCAATGCGGAGAAT

GTCCAACTGAATGCTTCCCCTGCGGTGAAGGAAACTGATAAGTCAAAAATAAACCCCCCACACGACAACA

ACTCACTTGATGATAAATACATCATGCCTTCAGATGATTTCTCAAACACTTTCTTCCCGCACGACACTGA

TCGCTTGAATTATCACGCAGATCATTTAGGTGATTATGACCTCGAAACCCTGTGTGAAGAGTCGGTTCTA

ATGGGAGTGATCAACTCTATAAAATTAATTAATCTGGATATGCGCTTAAATCACATTGAAGAACAAGTTA

AAGAGATCCCAAAGATCATCAATAAGCTTGAGTCCATTGACAGAGTTCTGGCCAAGACTAACACCGCACT

CTCAACCATTGAAGGACACCTGGTTTCTATGATGATAATGATACCAGGGAAAGGGAAAGGAGAAAGAAAG

GGGAAAAATAATCCTGAGCTTAAACCAGTGATAGGAAGAGACATTCTAGAGCAGCAATCTCTTTTTTCTT

TCGACAATGTCAAGAATTTCAGAGATGGATCGTTGACAAACGAACCGTATGGAGCAGCTGTACAGTTGAG

AGAAGATCTTATTCTTCCTGAACTTAATTTTGAGGAGACAAATGCATCTCAATTTGTTCCTATGGCAGAT

GATTCATCCAGGGATGTTGTCAAGACATTGATAAGGACTCACATTAAAGATAGAGAGTTGAGATCAGAAC

TGATTGGTTACCTGAATAAAGCGGAAAATGATGAGGAAATTCAGGAGATAGCAAACACTGTCAATGACAT

CATTGACGGTAACATTTGAATGGAGCCGGACATCAAGAGTATTTCAAGTGAGTCAATGGAAGGAGTATCT

GATTTCAGCCCTAGTTCTTGGGAGCATGGTGGGTATCTTGATAAGGTTGAACCAGAAATTGATGAAAATG

GCAGTATGATTCCAAAATACAAGATCTATACCCCAGGAGCTAACGAGAGGAAATACAACAACTACATGTA

CCTTATATGTTACGGCTTTGTTGAAGATGTTGAGAGAACCCCAGAGACAGGGAAACGCAAGAAGATCAGG

ACAATTGCTGCCTACCCTCTGGGTGTTGGTAAGAGTGCCTCTCATCCCCAAGATCTTCTGGAGGAACTCT

GTTCCCTCAAAGTTACTGTGAGGAGAACAGCTGGATCAACTGAGAAAATTGTGTTTGGATCATCTGGCCC

TCTAAATCACCTCGTTCCGTGGAAGAAAGTACTGACTGGTGGTTCAATTTTTAATGCAGTCAAGGTTTGT

CGGAACGTTGATCAGATACAGCTCGACAAGCATCAAGCTCTGAGAATATTTTTTCTCAGTATCACAAAGC

TCAATGATTCTGGAATCTACATGATTCCACGAACCATGCTTGAGTTCAGGAGAAACAATGCCATTGCCTT

CAATCTTCTAGTGTACTTGAAGATTGATGCTGATTTATCCAAAATGGGGATCCAGGGAAGCCTCGATAAA

GATGGCTTCAAGGTTGCCTACTTCATGCTACACTTGGGGAACTTTGTCCGTCGTGCAGGGAAGTATTACT

CTGTTGATTATTGTAGGAGGAAGATTGATAGGATGAAATTGCAGTTTTCACTGGGTTCCATAGGCGGACT

AAGTCTCCACATTAAGATCAATGGTGTAATCAGCAAACGGCTGTTTGCTCAAATGGGATTCCAAAAAAAC

CTTTGTTTCTCCTTGATGGACATCAATCCTTGGCTCAACAGATTGACCTGGAACAACAGTTGTGAGATCA

GCCGAGTAGCAGCTGTGTTGCAGCCTTCTGTTCCAAGAGAGTTCATGATCTATGATGATGTCTTCATTGA

CAATACAGGGAGAATTCTAAAGGGCTAAATGGTAGTTATACTTGACAAGAGATGTTATTCTAATCTTTTA

ATATTGATTTTGATGATCTCGGAGTGTAGTGTTGGGATTCTACATTATGAGAAATTGAGTAAAATTGGAC

TTGTCAAAGGAGTAACAAGAAAATACAAGATTAAAAGCAATCCTCTCACAAAAGACATTGTTATAAAAAT

GATTGCGAATGTGTCGAACATGTCTCAGTGCACAGGGAGTGTCATGGAAAATTATAAAACACGATTAAAC

GGTATCTTAACACCTATAAAGGGAGCGTTAGAGATCTACAAAAACAACACTCATGACCTTGTCGGTGATG

TGAGATTAGCCGGAGTTATAATGGCAGGAGTTGCCATTGGGATTGCAACCGCAGCTCAAATCACTGCAGG

TGTAGCACTATATGAGGCAATGAAGAATGCTGACAACATCAACAAACTCAAAAGCAGCATTGAATCAACT

AATGAAGCTGTCGTTAAACTTCAAGAGACTGCAGAAAAGACAGTCTATGTGCTGACTGCTCTACAGGATT

ACATTAATACTAATTTGGTACCGACAATTGACAAGATAAGCTGCAAACAGACAGAACTCTCACTAGATCT

GGCATTATCAAAGTACCTCTCTGATTTGCTTTTTGTATTTGGCCCCAACCTTCAAGACCCAGTTTCTAAT

TCAATGACTATACAGGCTATATCTCAGGCATTCGGTGGAAATTATGAAACACTGCTAAGAACATTGGGTT

ACGCTACAGAAGACTTTGATGATCTTCTAGAAAGTGACAGCATAACAGGTCAAATCATCTATGTTGATCT

AAGTAGCTACTATATAATTGTCAGGGTTTATTTTCCTATTCTGACTGAAATTCAACAGGCCTATATCCAA

GAGTTGTTACCAGTGAGCTTCAACAATGATAATTCAGAATGGATCAGTATTGTCCCAAATTTCATATTGG

TAAGGAATACATTAATATCAAATATAGAGATTGGATTTTGCCTAATTACAAAGAGGAGCGTGATCTGCAA

CCAAGATTATGCCACACCTATGACCAACAACATGAGAGAATGTTTGACGGGATCGACTGAGAAGTGTCCT

CGAGAGCTGGTTGTTTCATCACATGTTCCCAGATTTGCACTATCTAACGGAGTTCTGTTTGCCAATTGCA

TAAGTGTTACATGCCAGTGTCAAACAACAGGCAGGGCAATCTCACAGTCAGGAGAACAAACTCTGCTGAT

GATTGACAACACCACCTGTCCTACAGCCGTACTCGGTAATGTGATTATCAGCTTAGGGAAATATCTGGGG

TCAGTAAATTATAATTCTGAAGGCATTGCTATCGGTCCTCCAGTCTTTACAGATAAAGTTGATATATCAA

GTCAGAAATCCAGCATGAATCAGTCCTTACAACAGTCTAAGGACTATATCAAAGAGGCTCAACGACTCCT

TGATACTGTTAATCCATCATTAATAAGCATGTTGTCTATGATCATACTGTATGTATTATCGATCGCATCG

TTGTGTATAGGGTTGATTACATTTATCAGTTTTATCATTGTTGAGAAAAAGAGAAACACCTACAGCAGAT

TGGAGGATAGGAGAGTCAGACCTACAAGCAGTGGGGATCTCTACTACATTGGGACATAGATGCCGGCAGA

AAACAAGAAAGTTAGATTCGAAAATACTACTTCAGACAAAGGGAAAAATCCTAGTAAAGTTATTAAGAGC

TACTACGGAACCATGGACATTAAGAAAATAAATGAAGGATTATTGGACAGCAAAATATTAAGTGCTTTCA

ACACAGTAATAGCATTGCTTGGATCTATCGTGATCATAGTGATGAATATAATGATCATCCAAAATTACAC

AAGATCAACAGACAATCAGGCCGTGATCAAAGATGCGTTGCAGGGTATCCAACAGCAGATCAAAGGGCTT

GCTGACAAAATCGGCACAGAGATAGGGCCTAAAGTATCACTGATTGACACATCCAGTACCATTACTATCC

CAGCTAACATTGGGCTGTTAGGTTCAAAGATCAGCCAGTCGACTGCAAGTATAAATGAGAATGTGAATGA

AAAATGCAAATTCACACTGCCTCCCTTGAAAATCCACGAATGTAACATTTCTTGTCCTAACCCACTCCCC

TTTAGAGAGTATAGGCCGCAGACAGAAGGGGTGAGCAATCTGGTAGGATTACCTGATAATATTTGCCTGC

AAAAGACATCTAATCAGATACTGAAGCCAAAGCTGATTTCATACACTTTACCCGTAGTCGGTCAAAGTGG

TACCTGTATCACAGACCCATTGCTGGCTATGGACGAGGGCTATTTTGCATATAGCCACCTGGAAAGAATC

GGATCATGTTCAAGAGGGGTCTCCAAACAAAGAATAATAGGAGTTGGAGAGGTACTAGACAGAGGTGATG

AAGTTCCTTCTTTATTTATGACCAATGTCTGGACCCCACCAAATCCAAACACCGTTTACCACTGTAGTGC

TGTATACAACAATGAATTCTATTATGTACTTTGTGCAGTGTCAACTGTTGGAGACCCTATTCTGAATAGC

ACCTACTGGTCCGGATCTCTAATGATGACCCGTCTAGCTGTGAAACCCAAGAGTAATGGTGGGGGTTACA

ATCAACATCAACTTGCCCTACGAAGTATCGAGAAAGGGAGGTATGATAAAGTTATGCCGTATGGACCTTC

AGGCATCAAACAGGGTGACACCCTGTATTTTCCTGCTGTAGGATTTTTGGTCAGGACAGAGTTTAAATAC

AATGATTCAAATTGTCCCATCACGAAGTGTCAATACAGTAAACCTGAAAATTGCAGGCTATCTATGGGGA

TTAGACCAAACAGCCATTATATCCTTCGATCTGGACTATTAAAATACAATCTATCAGATGGGGAGAACCC

CAAAATTGTATTCATTGAAATATCTGATCAAAGATTATCTATTGGATCTCCTAGCAAAGTCTATGATTCT

TTGGGTCAACCTGTTTTCTACCAAGCGTCATTTTCATGGGATACTATGATTAAATTTGGAGATGTTCAAA

CAGTCAACCCTCTGGTTGTCAATTGGCGTGATAACACGGTAATATCAAGACCCGGGCAATCACAATGCCC

TAGATTCAATACATGTCCAGAGATCTGCTGGGAAGGAGTTTATAATGATGCATTCCTAATTGACAGAATC

AATTGGATAAGCGCGGGTGTATTCCTTGACAGCAATCAGACCGCAGAAAATCCTGTTTTCACTGTATTCA

AAGATAATGAAATACTTTATAGGGCACAACTGGCTTCTGAGGACACCAATGCACAAAAAACAATAACTAA

TTGCTTTCTCTTGAAGAATAAGATTTGGTGCATATCATTGGTTGAGATATATGACACAGGAGACAATGTC

ATAAGACCCAAACTATTCGCGGTTAAGATACCAGAGCAATGTACATAAATGGCCGACGAATTATCAATAT

CCGACATCATTTACCCTGAATGTCATTTGGATAGTCCTATAGTCTCTGGTAAACTAATATCAGCTATTGA

ATATGCTCAATTGAGACACAATCAACCCAGTGATGATAAAAGACTGTCTGAGAATATTAGGTTAAACCTT

CACGGGAAAAGAAAGAGTCTATACATATTAAGACAATCCAAACAGGGTGATTACATTAGAAACAACATAA

AAAACCTAAAGGAATTCATGCATATTGCGTACCCTGAATGCAATAACATTCTATTCTCCATCACATCCCA

AGGCATGACTAGCAAACTTGATAACATCATGAAAAAGTCATTCAAAGCATACAATATCATTAGTAAGAAA

GTAATTGGGATGCTGCAAAATATCACTAGAAATCTCATAACTCAAGATAGAAGAGATGAAATAATTAATA

TACATGAGTGTAGGCGATTAGGGGATTTAGGGAAGAATATGAGTCAATCTAAATGGTATGAGTGTTTTTT

GTTTTGGTTTACTATCAAAACAGAGATGCGAGCAGTGATCAAGAATTCGCAAAAGCCGAAATTCCGTTCA

GATTCATGCATAATACACATGCGAGACAAAAGTACTGAAATAATCCTAAATCCGAATCTTATCTGCATTT

TCAAATCAGACAAAAATGGAAAGAAGTGTTATTATCTTACACCCGAAATGGTTCTAATGTATTGTGATGT

CCTAGAGGGAAGGATGATGATGGAGACAACAGTCAAATCGGATATCAAGTACCAACCTCTAATCTCGAGA

TCCAATGCCCTCTGGGGGCTAATTGATCCCTTGTTCCCTGTCATGGGAAACAGAATTTACAATATAGTGT

CTATGATAGAGCCTTTAGTTCTTGCACTACTCCAACTCAAGGATGAGGCTAGGATCCTGAGGGGTGCATT

TCTGCATCACTGCATAAAGGAAATGCATCAAGAATTGAGTGAGTGTGGTTTTACAGATCAGAAGATTCGG

TCTATGTTTATTGATGATCTTTTATCCATTCTAAATATCGATAATATACATCTGTTGGCAGAGTTCTTTT

CTTTCTTTCGTACGTTTGGCCATCCTATTCTTGAGGCTAAAGTTGCTGCAGAAAAAGTGAGAGAACATAT

GTTGGCAGATAAAGTTCTTGAATATGCCCCTATAATGAAAGCACATGCTATATTCTGCGGGACTATAATA

AATGGGTATAGGGATAGACACGGAGGAGCCTGGCCTCCTCTTTACCTCCCCGCACATGCATCTAAACATA

TAATCCGTTTGAAAAATTCTGGGGAATCTTTGACCATTGATGACTGTGTCAAGAATTGGGAATCATTCTG

TGGGATTCAATTTGATTGTTTCATGGAGCTGAAATTGGACAGTGATCTGAGTATGTATATGAAAGATAAA

GCTTTATCTCCAATCAAAGACGAATGGGACAGTGTATACCCACGTGAAGTGTTGAGCTATACCCCACCGA

AGTCAACCGAGCCAAGAAGATTGGTTGACGTTTTTGTAAATGATGAAAACTTTGATCCATACAACATGCT

GGAATATGTCTTATCCGGTGCTTATCTCGAGGATGAACAATTCAATGTTTCTTATAGCTTGAAGGAGAAA

GAGACGAAGCAAGCTGGACGATTGTTCGCAAAGATGACCTACAAAATGCGTGCATGTCAAGTCATAGCAG

AGGCCCTGATAGCCTCAGGTGTCGGTAAATATTTTAAGGAGAACGGGATGGTTAAGGATGAGCACGAACT

TTTGAAGACACTCTTCCAATTGTCTATTTCCTCAGTTCCTCGAGGGAACAGTCAGGGTAATGATCCTCAA

TCCATCAATAATATAGAAAGAGATTTCCAATACTTTAAAGGGGTCACTACCAATGTGAAAGACAAAAAGA

ATAACTCTTTTAATAAGGTTAAATCTGCTCTCAATAATCCGTGCCAAGCTGACGGAGTCCATCATAACAT

GTCACCCAATACACGAAATCGTTATAAGTGTAGTAATACAAGTAAGTCTTTTCTCGATTATCATACCGAG

TTTAATCCTCACAATCACTATAAATCAGACAATACAGAGGCGGCCGTACTGTCCAGGTATGAGGACAACA

CTGGGACAAAATTTGATACAGTAAGTGCATTTCTTACAACTGATCTTAAGAAATTCTGTCTCAATTGGAG

ATACGAATCAATGGCTATATTTGCTGAACGTCTAGATGAGATATACGGTTTACCTGGATTTTTTAATTGG

ATGCACAAACGACTAGAAAGATCTGTTATCTATGTTGCAGACCCTAATTGCCCCCCTAATATTGACAAAC

ATATGGAACTAGAAAAAACTCCTGAAGATGATATATTCATTCATTATCCTAAAGGCGGTATTGAAGGATA

TAGCCAAAAAACATGGACTATAGCAACTATCCCCTTTTTATTCTTGAGTGCCTATGAGACAAACACGAGG

ATTGCTGCAATTGTCCAAGGAGACAATGAATCAATTGCTATCACTCAAAAAGTTCATCCTAATCTTCCTT

ACAAGGTAAAGAAAGAGATCTGTGCAAAGCAAGCTCAGCTTTATTTTGAAAGGTTAAGGATGAACTTAAG

AGCCCTCGGCCACAATCTTAAAGCTACAGAAACTATCATCAGTACACATCTTTTTATTTATTCGAAGAAA

ATTCATTATGATGGTGCTGTGCTGTCTCAGGCACTCAAATCAATGTCAAGATGTTGCTTTTGGTCAGAGA

CTCTGGTGGATGAAACTAGATCAGCTTGTAGTAACATCAGCACTACAATAGCTAAAGCTATAGAAAATGG

GTTGTCAAGAAATGTCGGCTATTGCATCAATATTTTGAAAGTAATTCAGCAGCTTCTCATATCAACTGAG

TTTAGTATTAACGAGACATTGACACTGGATGTGACATCTCCCATTTCAAATAATTTAGATTGGCTTATAA

CAGCTGCATTAATCCCGGCACCTATTGGAGGATTCAATTACCTTAATTTGTCTAGAATTTTTGTTAGAAA

TATAGGTGATCCGGTTACAGCATCTTTGGCTGATCTTAAGAGAATGATTGATCACAGTATTATGACTGAA

AGCGTATTACAAAAAGTTATGAATCAAGAACCTGGTGATGCGAGTTTCTTGGACTGGGCCAGTGATCCAT

ACTCGGGCAACTTGCCTGACTCACAAAGCATCACTAAAACAATTAAAAATATCACAGCAAGGACTATACT

GAGGAACTCACCGAACCCAATGCTAAAAGGTTTATTTCATGACAAATCTTTTGATGAAGATCTTGAACTA

GCTAGCTTCTTAATGGACAGGAGGGTTATATTACCTAGAGCCGCTCATGAGATACTGGATAATTCATTGA

CAGGTGCCAGAGAGGAAATTGCTGGTTTATTAGATACAACTAAAGGCTTGATCAGATCAGGGCTAAGAAA

GAGTGGACTTCAGCCAAAGTTAGTTTCTAGATTATCTCATCATGATTATAATCAATTTTTAATACTGAAC

AAACTTCTATCAAACAGAAGACAAAATGACTTGATATCATCAAATACTTGCTCAGTTGACTTGGCACGAG

CATTGAGATCTCACATGTGGAGGGAATTAGCGTTAGGTAGAGTAATATACGGTCTTGAGGTACCGGATGC

ACTTGAGGCTATGGTGGGAAGGTATATAACAGGGAGCTTAGAGTGCCAAATTTGTGAGCAGGGAAACACG

ATGTATGGGTGGTTCTTTGTACCTAGGGATTCCCAATTGGATCAGGTAGATAGAGAGCACTCATCAATAA

GAGTACCTTATGTAGGATCAAGTACGGATGAAAGATCGGATATCAAACTAGGGAATGTCAAAAGACCAAC

TAAGGCCTTGCGTTCTGCTATCAGAATTGCGACAGTATATACTTGGGCCTATGGGGACAATGAAGAGTGT

TGGTATGAAGCTTGGTACCTAGCGTCTCAGAGGGTAAACATAGACTTAGATGTATTGAAAGCTATAACCC

CAGTTTCCACTTCAAACAATTTATCCCATAGATTGAGAGATAAATCCACACAATTTAAGTTTGCAGGGAG

TGTACTCAACAGAGTTTCTAGATATGTTAACATAAGCAATGACAATCTAGATTTCAGAATTGAGGGAGAA

AAGGTAGATACGAATCTTATTTATCAACAAGCAATGCTATTAGGGTTATCGGTATTGGAAGGTAAATTCA

GATTGAGATTAGAAACTGATGATTACAACGGGATATATCACTTACACGTAAAGGATAATTGTTGTGTCAA

AGAAGTGGCTGATGTAGGCCAAGTAGACGCTGAGTTGCCTATCCCAGAATATACTGAAGTGGATAACAAT

CATCTTATATATGATCCAGACCCCGTTTCAGAAATAGATTGCAGCCGTCTTTCTAATCAGGAGTCCAAAT

CAAGAGAATTAGACTTTCCTTTATGGTCAACTGAGGAACTTCATGATGTCCTAGCTAAGACTGTTGCTCA

GACCGTTCTTGAGATTATAACAAAGGCTGACAAGGATGTTTTAAAGCAACACCTTGCAATAGACTCTGAC

GATAACATCAACAGCTTAATCACAGAATTTCTAATAGTTGATCCTGAACTGTTTGCACTTTATCTAGGAC

AATCTATATCAATAAAATGGGCCTTTGAAATTCATCATAGGCGTCCTAGAGGAAGACATACTATGGTCGA

CCTATTGTCAGATCTTGTATCAAATACATCAAAGCACACTTACAAAGTGTTGTCAAATGCCTTGTCACAT

CCTAGAGTATTCAAGAGATTTGTAAACTGTGGCTTGCTATTGCCTACACAGGGTCCTTACCTTCATCAAC

AAGATTTTGAAAAGTTGTCTCAAAACCTTCTTGTAACATCTTATATGATTTATCTAATGAACTGGTGTGA

CTTCAAGAAATTCCCCTTTTTAATCGCCGAACAGGATGAAACTGTGATAAGTCTACGAGAGGATATAATA

ACATCCAAACATCTCTGTGTTATAATTGACTTATATGCAAATCACCATAAACCTCCTTGGATAATAGATC

TAAACCCACAAGAAAAAATATGTGTACTGCGTGACTTTATTTCTAAATCTAGGCATGTGGACACGTCCTC

CAGATCATGGAATACTTCTGACCTGGATTTTGTAATATTCTATGCATCTTTGACTTATTTGAGAAGAGGT

ATAATAAAACAATTAAGGATAAGACAAGTTACTGAGGTTATAGATACCACAACAGTGTTAAGGGATAATA

TAATTGTAGAGAATCCTCCTATTAAAACAGGAGTGTTAGACATCAGAGGTTGTATAATATACAATTTAGA

GGAAATCCTGTCTATGAACACAAAATCAGCATCAAAAAAGATCTTTAATCTTAATAGTAGGCCGTCAGTG

GAGAATCATAAATATAGAAGGATAGGTCTCAACTCATCATCTTGTTACAAGGCATTAAATCTATCACCTC

TGATTCAAAGGTATTTGCCGTCGGGAGCTCAAAGGTTGTTTATAGGAGAAGGTTCTGGGAGCATGATGTT

ATTATATCAGTCTACATTGGGGCAATCAATTTCTTTTTACAATTCAGGTATAGATGGAGATTATATACCA

GGTCAAAGAGAACTGAAACTATTTCCCTCTGAATACTCAATTGCTGAGGAAGACCCATCTCTGACGGGGA

AATTGAAAGGACTAGTGGTGCCCCTATTCAATGGAAGACCAGAAACAACATGGATCGGGAATTTAGACTC

CTACGAGTATATCATAAATAGGACAGCGGGGCGAAGTATAGGTCTTGTCCATTCTGACATGGAGTCTGGG

ATTGACAAAAATGTAGAGGAGATACTAGTAGAACATTCCCATCTAATATCTATCGCGATAAATGTTATGA

TGGAGGACGGACTATTAGTATCCAAGATAGCATACACCCCTGGATTCCCAATCTCAAGATTATTTAACAT

GTACAGATCATATTTCGGACTAGTACTGGTGTGTTTCCCAGTATATAGTAATCCAGATTCTACTGAAGTA

TATCTTCTTTGCTTACAGAAGACGGTCAAGACTATTGTTCCCCCGCAAAAAGTCCTTGAGCACTCTAATT

TGAACGATGAAGTCAATGACCAGGGAATAACATCAGTGATTTTTAAAATCAAGAATTCACAGTCTAAGCA

GTTCCACGATGATCTAAAGAAGTACTATCAGATTGACCAACCTTTTTTTGTACCAACTAAAATCACTAGT

GATGAACAAGTACTTCTCCAAGCAGGGCTGAAACTCAATGGGCCAGAAATTCTTAAGAGTGAAATCAGTT

ATGATATCGGTTCAGATATCAATACATTAAGAGACACCATCATAATTATGTTAAATGAGGCTATGAATTA

TTTTGATGACAACAGATCACCTTCACACCACCTAGAACCCTATCCAGTTTTGGAGAGAACTAGAATTAAA

ACAATAATGAATTGTGTGACTAAGAAAGTGATTGTCTACTCACTTATCAAGTTCAAGGACACCAAAAGCT

CAGAACTTTATCACATCAAAAATAACATCAGAAGAAAAGTTCTAATCTTAGATTTCAGATCGAAGCTCAT

GACAAAAACTCTACCTAAAGGGATGCAAGAGAGAAGAGAAAAAAACGGTTTCAAAGAAGTTTGGATAGTA

GATTTATCGAATCGAGAAGTTAAAATCTGGTGGAAGATAATCGGATACATATCTATTATCTGA