Effect of Metformin Hyperbaric O2 Therapy and Supplements Cocktail

Need to write the methodology part of the thesis.
Five groups of mice
7 in each group ( should mention the statical significance of the sample size )group
first group is control group
3 group will be expose to different anti-aging medicine
the last group will receive all
biological age will be measured by telomere length, inflammatory markers, and cellular senescence by tissue biopsy.